FIG. 1.

Experimental setup and S1PR agonist diffusion model. (A) Microfluidic device. (B) Schematic of outlined area in (A), showing proximal (top image) and distal (bottom image) sphingosine 1-phosphate (S1P) receptor agonist gradients (red, high concentration; blue, low concentration; green ovals, cells). (C) Computational model of static S1PR agonist diffusion profile at 12 h (left image) and concentration versus distance plot (right image) for t=0–12 h. (D) Diffusion profile of S1PR agonist at 12 h (left image) and concentration versus distance plot (right image) for t=0–12 h under 1 μL/min flow.