FIG. 6.

Distal S1P1/3 stimulation under flow promotes stable endothelial sprout formation. (A) Schematic of distal S1PR agonist stimulation in the presence of VEGF and 1 μL/min flow. (B) Sprout density for arterial (left) and venular (right) cells under flow, normalized to static basal media (value of unity, bar not shown). (C) Sprout-to-migration ratio (A.U.) for HAAECs (left) and HUVECs (right) under flow normalized to flow basal media. (D) Representative images of sprouting and migration data shown in (B, C). Magnification=20×. Green color indicates GFP. Error bars represent standard error. *p<0.05. Color images available online at www.liebertpub.com/tea