FIG. 5.

Altered gene expression in mESC-embedded collagen scaffolds following exposure to cyclic stretching, as determined using a TaqMan^® Array Custom Micro Fluidic Card. (A) Fold change (log scale) in expression of genes representing the three germ layers upregulated or downregulated by >20% in at least three of four experiments. (B) Fold change (log scale) in expression of genes associated with cell stemness or pluripotency upregulated or downregulated by >20% in at least three of four experiments. (C) A gene enrichment map of all the genes in the array that were downregulated or upregulated. (D) Cellular processes related to the resulting gene expression profile. Processes related to mesodermal differentiation are presented. Color images available online at www.liebertpub.com/tea