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. 2014 Sep 26;21(1-2):332–342. doi: 10.1089/ten.tea.2014.0003

FIG. 2.

FIG. 2.

Histological and immunohistochemical findings in explanted native (A–D) and decellularized (E–J) porcine 9-mm-diameter aortic roots following 6 weeks of implantation in the descending abdominal aorta of pigs. (A) H&E-stained section of native explant showing the formation of a neointima and adventitia with destruction of the implanted tissue (×40). (B) Immunostaining for von Willebrand factor showing the formation of a continuous endothelium (×40). (C) Von Kossa-stained section showing extensive calcification of the implanted native tissue (×40). (D) Immunostaining for CD3 indicating the presence of T cells around the native tissue (×100). (E) H&E-stained section of decellularized explant showing the presence of cells within the tissue (×100). (F) Von Kossa-stained section of the suture point (×100). (G) Immunostaining for α-smooth muscle actin (α-SMA) indicating that the majority of cells are smooth muscle cells (×40). (H) Immunostaining for vimentin showing the extensive presence of aligned cells in the implanted tissue (×40). (I, J) Immunostaining for MAC clearly showing a wall of macrophages at the interface between the neointima and the implanted tissue from both explants highlighted by the white arrow (×40). Black arrows indicate the direction of aortic lumen. H&E, hematoxylin and eosin.