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. 2014 Sep 26;21(1-2):332–342. doi: 10.1089/ten.tea.2014.0003

FIG. 4.

FIG. 4.

Histological and immunohistochemical findings in explanted native ovine 15-mm-diameter aortic roots following 6 months implantation in the right ventricular outflow tract of juvenile sheep. (A) H&E-stained section of explanted root ES3 showing extensive damage to the root wall (×40). (B) Elastin van Gieson-stained longitudinal sections of aortic roots ES3 (upper) and ES7 (lower) showing that the damage to the wall extended the lengths of the implants and showing the thickened retracted leaflets (×4). (C) Von Kossa-stained cross-sectional view of aortic root ES1 showing that calcification was present throughout (×4). (D) Von Kossa-stained longitudinal section of aortic root ES3 showing calcification of the wall (×4). (E) Immunostaining for CD3 in aortic root wall of ES3 showing T-cell infiltrate (arrow) (×40). (F) Immunostaining for CD3 in leaflet of root ES3 showing T-cell infiltrate (arrow) (×40). (G) Immunostaining for Ki-67 in aortic root wall of ES3 showing the presence of proliferative cells (arrow) (×40). (H) Immunostaining for MAC in aortic root wall of ES1 showing the absence of macrophages (×40).