FIG. 5.

Low-power overviews of the histology of explanted decellularized porcine 15-mm-diameter aortic roots following 6 months implantation in the right ventricular outflow tract of juvenile sheep. (A–C) Elastin van Gieson-stained longitudinal sections of aortic roots EDP1, EDP3, and EDP7, respectively, showing integration and remodeling of the aortic walls and thin leaflets. (D–F) Von Kossa-stained longitudinal sections of aortic roots EDP1, EDP3, and EDP7, respectively, showing the location of calcium deposits at the suture points of all roots and evidence of some calcification in the central region of the wall of aortic root EDP3. Black arrows indicate suture points and red arrows indicate leaflets.