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. 2015 Jan 13;10(1):e0115979. doi: 10.1371/journal.pone.0115979

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© 2015 Sun et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The expression level of the target gene hsp83 was determined at different temperature. Three recommended reference genes (A, 18S rRNA; B, RPS20; and C, TUB) and the least stable gene (D, GAPDH) were used as reference genes in qRT-PCR. The data represent the mean ± SE. Different letters above vertical bars represent significant differences (P = 0.05) (Tukey’s test).