Table 1. List of primers used in this study.
Primer | Sequence a |
---|---|
pr2Fn | GAGCTCGGTACCCGGGGATCATGGACGAGCCACTCGTATC |
pr2R | CAGGTCGACTCTAGAGGATCCATTCATCTATTTCTCCCTCTCTTC |
ermF1 | TGGATCCTCTAGAGTCGACCTGCAGACGTATATAGATTTCATAAAGTC |
ermR1 | TTACGCCAAGCTTGCATGCCTGCAGCATTCCCTTTAGTAACGTGTAAC |
repF | GAAATCAACGCCCATACGTC |
repR | TATCGTCTGCACACCGTTTC |
tufAF | GGTAGTTGTCGAAGAATGGAGTGTGA |
tufAR | TAAACCAGGTTCAATCACTCCACACA |
mcdMF | CGGAATTCAGTTCTTTCTACGG |
mcdMR | GCTTCACCAATAAGCGTTCC |
reb1F | GGACGGAGCATTGACTCTATTG |
reb1R | CTGTTCCTGCAAATTTTCCAAC |
mobb1F | ATTGGGTTCTGATTTTGGAAGG |
mobb1R | CACCTAATCCAAGAACGACTGC |
M13F | GTAAAACGACGGCCAGT |
M13R | CAGGAAACAGCTATGAC |
aAll primers were designed during this study except from primers tufAF/tufAR that were designed in [55] and primers M13F/M13R that hybridize with the pUC19 backbone.