Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Mol Neurosci. 2014 Aug 10;55(1):152–159. doi: 10.1007/s12031-014-0397-1

Figure 2. PMA and LIF reduce pSTAT3 levels but have no effect on SOCS3, PIAS3 or STAT3 levels.

Figure 2

(A) Western blot showing SOCS3 protein levels found in PN1 retinas after thirty minute treatment with 100nM PMA, 20ng/mL LIF or 50ng/mL IGF1. (B) Average amount of total SOCS3 protein found in three separate PN1 retinas treated as in A (n=3). (C) Western blot showing PIAS3 levels after 30 min treatment with 50 ng/ml IGF1 or 100 nM PMA. (D) Average amount of total PIAS3 protein found in PN1 retinas treated as in C (n=5). (E & F) the retinas in C and D showed no change in total STAT3 levels (E) but significant reductions in pSTAT3 level (F). * P<0.05, ** P<0.005 vs control.