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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Dec 11;57:20–26. doi: 10.1016/j.ibmb.2014.12.003

Fig. 2. The inhibitory effect of AeaAST-C is reversible.

Fig. 2

Br-CA-CC were incubated for two consecutive 2 h periods. A) During the first 2 h, Br-CA-CC complexes were incubated either in the presence or absence of AeaAST-C (10−9 M). After 2 h, glands were rinsed in fresh culture medium. B) During the second 2 h period, control and previously treated glands were incubated in fresh culture medium with and without AeaAST-C. Each data point represents the mean ± S.E.M. of three independent determinations of three Br-CA-CC complexes. Asterisks denote significant differences (unpaired t-test; ***P<0.0001). Different letters above the columns indicate significant differences among treatments (ANOVA P<0.05, with Tukey’s test for multiple comparison).