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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Dec 11;57:20–26. doi: 10.1016/j.ibmb.2014.12.003

Fig. 5. Stimulation with precursors overrides the inhibitory effect of AeaAST-C.

Fig. 5

The effect of AeaAST-C (10−9 M) on JH biosynthesis was evaluated on Br-CA-CC complexes incubated in culture medium M-199 alone (control) or with the addition of 200 μM of nine different precursors: ACoA (acetyl-CoA), MVA (mevalonic acid), MevP (mevalonic acid 5-phosphate), MevPP (mevalonic acid 5-pyrophosphate), FPP (farnesyl diphosphate), FOL (farnesol), FAL (farnesal), FA (farnesoic acid) and MF (methyl farnesoate). Each data point represents the means ± S.E.M. of 2-4 independent biological replicates of three Br-CA-CC complexes. Asterisks denote significant differences (unpaired t-test; *** P ≤ 0.001).