Figure 2. Functional expression of Cn Yap1 in S. cerevisiae.
A. A S. cerevisiae strain designated SM12 (relevant genotype: yap1Δ leu2::ARE-TRP5-lacZ-LEU2) was transformed with a high-copy- (2µm Sc YAP1) or low-copy-number (CEN Sc YAP1) plasmid containing wild-type Sc YAP1, a low-copy-number plasmid carrying the CUP1-Cn YAP1-GFP fusion gene (CEN Cn YAP1) and an empty vector plasmid (Vector). Transformants were grown to mid-log phase and equal numbers of cells were then placed on media containing a gradient of the indicated oxidants (H2O2, diamide), drug (4-nitroquinoline-N-oxide: 4-NQO) or heavy metal (cadmium sulfate). Increasing concentration along the gradient is indicated by the bar of increasing width. Plates were incubated at 30°C for 48 hours and then photographed. B. Transformants from above were grown to mid-log phase and then β-galactosidase activity determined as described before (31).