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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Fungal Genet Biol. 2014 Nov 1;74:1–9. doi: 10.1016/j.fgb.2014.10.015

Figure 3. Regulated nuclear localization of Cn Yap1 in S. cerevisiae.

Figure 3

Plasmids expressing an irrelevant GFP fusion protein (pJPS10) or GFP fusion proteins forms of either Cn Yap1 (pSP64) or Sc Yap1 (pNT13) were introduced into S. cerevisiae cells containing a yap1Δ allele. Appropriate transformants were grown to mid-log phase and then incubated in the presence of DAPI for 30 minutes. Aliquots were withdrawn (0 time point) and then 1 mM H2O2 added with incubation continued for 20 minutes. Cells were visualized by fluorescence microscopy for GFP and DAPI or by Nomarski optics (DIC).