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. Author manuscript; available in PMC: 2016 Jan 12.
Published in final edited form as: Dev Cell. 2014 Dec 18;32(1):19–30. doi: 10.1016/j.devcel.2014.11.015

Figure 6. RpS6 targeting by PALL promotes RAC up-regulation and activation.

Figure 6

(A) WB of protein extracts from mock-treated, pall- or RpS6-RNAi S2 cells probed with Tubulin and monomeric Actin (internal controls), and mammalian RAC1 Abs. (B) Graph showing the % ± SEM of RAC protein expression in pall- and RpS6-RNAi S2 cells relative to mock-treated cells (control normalized at 100%) based on three separate experiments. (C) Confocal micrographs of mock-treated, pall- and RpS6-RNAi S2 cells stained with active RAC Ab. Z-stack images through the cells were collected at 1.74 μm intervals. Scale bars correspond to 10 μm. (D) Graph showing the mean PIs ± SEM of crq-gal4 control-, crq-gal4;pallko mutant- and pallko-macrophages that overexpress UAS-Rac2 under the control of crq-gal4. See also Fig. S5.