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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Eur J Immunol. 2014 Oct 27;45(1):298–308. doi: 10.1002/eji.201444911

Figure 2. CD25+PD1+CXCR5+CD4+ T cells are not Treg cells.

Figure 2

(A) Tonsil CD4+ T cells were purified, stained and analyzed by flow cytometry. (B) Tonsil cells were gated on CD4 expression to determine the percentage of FoxP3+ cells in PD1−CD25+ (58.9%), PD1−CD25− (1.2%), PD1+CD25+ (2.8%) or PD1+CD25− (0.9%). Data are summarized in a bar graph showing mean (+SEM) percentages of FoxP3+cells (n=7 donors) and are representative of 3 independent experiments. P < 0.05 was considered to be significant; Student’s t test.