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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Eur J Immunol. 2014 Oct 27;45(1):298–308. doi: 10.1002/eji.201444911

Figure 7. CD25+ Tfh cells are incorporated preferentially into T:B-cell conjugates.

Figure 7

(A and B) Total tonsil cells were stained with CD4, CD19, PD1 and CD25. Relative cell size based on (A) scattered light and (B) CD25/PD1 expression of different subsets was analyzed by flow cytometry. CD4 and CD19 double positive cells were purified by cell sorting. (C) Cell size is evaluated based on scattered light. (D) CD19+ (B cells) or CD4+ (Tfh cells) expression before and after sorting.(E–J) Expression of (E) CD38, (F) AID, (G) CD45RO, (H) IgG, (I) IgA and (J) MHCII on B cells conjugated with CD25+and CD25− Tfh cells were analyzed by flow cytometry. (A–J) Data are representative of three independent experiments each with different donors (n=5).