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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Glia. 2014 Oct 21;63(3):441–451. doi: 10.1002/glia.22763

Figure 1. HIV infection upregulates Wnt 2b, 10b, and 16 in PDAs.

Figure 1

(A) PDAs were infected with HIVBaL at 10ng per 1×106 cells for 24 hours or left uninfected. Six days later Wnt ligand mRNA transcript levels of each of the known 19 Wnt ligands were tested by qRT-PCR. Wnt 7b was the lowest detected ligand therefore all of the Wnt ligands were normalized to Wnt 7b. Each experiment was performed twice in triplicate. * indicates significance of p≤0.05 between uninfected and HIV infected groups using one way analysis of variance (ANOVA). (B) ACM was harvested from three day PDA cultures. Wnt ligands (Wnt 1, 2b, 3, 5b, and 10b) were removed by immunoprecipitation. Western blots were performed on 40μl of ACM. Image is representative of three separate experiments.