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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Glia. 2014 Oct 21;63(3):441–451. doi: 10.1002/glia.22763

Figure 2. Astrocytes (PDA) conditioned media (ACM) inhibits HIV infection in PBMCs.

Figure 2

Human PBMCs from four healthy donors were cultured at 2×106 cells/mL overnight with α-CD3/CD28 and IL-2. Cells were infected with HIVBaL at 2ng/1×106 cells for four hours. Infected PBMCs were supplemented with 0–100% ACM. ACM was prepared using supernatant from PDAs cultured for three days in completed DMEM with 10% FBS. ACM was diluted with serum free DMEM media for 0–90% ACM culture conditions. At day 6, MTS assays (A) and HIVp24 ELISA (B) were performed. Data is based on four different healthy donors and experiments were performed in triplicate. *indicates significance of p<0.05 between control and experimental group using one way ANOVA between RPMI control well and 75% and 100% groups. In (C), PBMCs were activated and infected as in (A) then cultured with complete DMEM, ACM, or ACM from HIV infected PDAs. ACM was prepared from 6 day PDA cultures. ACM from HIV infected cultures was ultracentifuged prior to transfer to PBMCs. HIV p24 ELISA from PBMCs was measured at day 6 post-transfer. * indicates significance of p<0.05 between control and experimental group using one way ANOVA between control and experimental groups.