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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Glia. 2014 Oct 21;63(3):441–451. doi: 10.1002/glia.22763

Figure 4. Conditioned Media from CD8+ T cells activates astrocytes.

Figure 4

CD8+ T cells were isolated from PBMCs from healthy seronegative donors by magnetic bead isolation and left untreated or treated with 1 μg/ml α-CD3/CD28 co-stimulation and 20U/mL IL-2. At day 3, supernatant from un-activated or activated CD8+ T cells was added to PDAs for three days. PDAs were analyzed by FACS analysis for surface expression of HLA-DR (A & B) and intracellular IFNγ (C & D). Data is representative of three experiments *p≤0.05 between DMEM media and treated groups, as measured by student’s t test.