Figure 1.

Expression of three genes from single polyprotein constructs. a, Three polyprotein constructs, each encoding a single ORF incorporating three different genes separated by FMDV 2A, were assembled and translated in a reticulocyte lysate in vitro. Arrows indicate the positions where the polyproteins would be expected to dissociate cotranslationally at the carboxy terminus of 2A. b, Products obtained when the polyprotein constructs are translated in vitro. Lane 1, Products of pGFP2A-CAT2A-GUS translation. Lane 2, Products of pGUS2A-CAT2A-GFP translation. Lane 3, products of pGFP2A-P42A-GUS translation.