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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Neurooncol. 2014 Sep 26;121(1):109–118. doi: 10.1007/s11060-014-1618-8

Fig. 5.

Fig. 5

EphrinB1 knockdown inhibits MB cell migration.

Scratch assay results of D556 cells transfected with negative control siRNA or ephrinB1 siRNA at 0, 24 and 48 h time points after scratch induced. a) Representative photomicrographs (10X) of scratch assay under light microscopy at the indicated time points show that ephrinB1 knockdown cells are impaired in their ability to migrate inwards to fill in the scratch at 24 and 48 h compared to negative control cells. b) Graph of the average distance migrated in cm over 24 h in scratch assays demonstrates that ephrinB1 knockdown cells have significantly decreased migration in comparison to negative control cells. Bars represent the mean ± SD of 3 separate experiments (*P=0.01)