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In vitro assessment of Glial Restricted Precursors (GRPs): Cells were derived at E13.5 and maintained in GRP culture. Cells were confirmed to be GRPs by their expression of A2B5 (A). As previously reported about 40% of the cells expressed Nestin (B). A week after culturing these immunopanned cells, almost all cells were PDGFR-a positive (C).
