Figure 2.

Elution of POMT from a Poros HQ column used for anion-exchange chromatography. Bound proteins were eluted in a 0- to 500-mm and 500- to 1,500-mm two-step linear NaCl gradient. The eluate was collected in 1-mL fractions; the flow rate was 6 mL min⁻¹. OMT activities were assayed in each fraction using three substrates (PLG, DHA, and DMP); they are shown as gray lines (see legend in the inset). Note that activities are given at different scales for the substrates (the lower, solid part of the axis is valid for the substrates DHA and DMP, while the upper, dotted part refers to the substrate PLG). Total protein contents were monitored by measuring the A₂₃₀. Two activity peaks are marked (arrows 1 and 2), one of which is highly specific for PLG (arrow 1).