Table I.
Purification Step | Total Protein | Total Activity | Specific Activity | Purification Ratio | Recovery |
---|---|---|---|---|---|
mg | pkat | pkat/mg protein | % | ||
Crude extract Sephadex (G-25) | 31.06 | 72.00 | 2.32 | 1.00 | 100.00 |
(NH4)2SO4 (30%–80%) | 10.78 | 24.80 | 2.30 | 0.99 | 34.44 |
Poros HQ | 0.34 | 16.40 | 47.81 | 20.63 | 22.78 |
Poros HQ | 0.19 | 13.00 | 67.71 | 29.21 | 18.06 |