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Kinetic parameters of purified native and recombinant POMT with PLG or SAM as substrate
| POMT (origin)
|
Km
|
Kcat
|
Kcat/Km
|
|||
|---|---|---|---|---|---|---|
|
μm
|
S−1 × 10−3
|
mm−1 × S−1
|
||||
| PLG | SAM | PLG | SAM | PLG | SAM | |
| Native | 14.40 ± 0.28 | 1.93 ± 0.16 | 4.49 ± 0.18 | 11.21 ± 0.54 | 0.31 | 5.81 |
| Cloned | 94.50 ± 3.78 | 16.15 ± 0.65 | 2.01 ± 0.08 | 5.46 ± 0.22 | 0.02 | 0.34 |
