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. 2015 Jan 6;10:305–320. doi: 10.2147/IJN.S73971

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© 2015 Abbad et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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In vitro evaluation of cellular internalization.

Notes: (A) Cellular uptake of free C6, C6-PNs, and C6-MNs with and without pretreatment with free HA polymer, in A549 as a function of the incubation time (2 and 4 hours at 37°C). (B) Confocal microscopic images of A549 cells after incubation with free C6, C6-PNs, and C6-MNs for 2 and 4 hours. (C) Quantitative flow cytometric analysis of the intracellular uptake of C6, C6-PNs, and C6-MNs with and without pretreatment with free HA polymer in A549 cells for 2, 4, and 6 hours of incubation. **^,##,××P<0.01. (D) Flow cytometric curves of C6, C6-PNs, and C6-MNs with and without pretreatment with free HA polymer after 4 hours of incubation in A549 cells. Each analysis was generated by counting 10⁴ cells. Results are expressed as the mean ± SD.

Abbreviations: C6, coumarin-6; C6-MNs, coumarin-6-loaded mixed nanoparticles; C6-PNs, coumarin-6-loaded plain nanoparticles; HA, hyaluronic acid; SD, standard deviation.