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Fluorescence microscopy images of rBMSC cultured on the glycerol-treated silk scaffolds after the removal of glycerol: (A) 1st day; (B) 6th day; (C) 12th day. The percentages inside images were the contents of glycerol before glycerol removal while SA and MA indicated salt-leached silk scaffolds and methanol-treated silk scaffolds without glycerol treatment. Blue (DAPI) for nuclei and silk fibroin scaffolds; green (FITC labeled phalloidin) for F-actin.
