Figure 1.

Immunoblot showing the specificity of YS1 antibodies. Blots were probed with affinity-purified anti-YS1 antibodies at a dilution of 1:100. A, Protein samples were prepared from roots and leaves of iron-starved wild-type (WT) and ys1-ref (ys1) mutant plants. B, Protein samples were prepared from roots of fully fed (+Fe) and iron-starved (−Fe) wild-type (WT) plants, and from fully fed (+Fe) ys1-ref mutant plants.