Figure 3.

Response to iron resupply in roots. Protein and RNA were prepared simultaneously from roots of maize seedlings grown hydroponically in iron-sufficient (+Fe) or iron-insufficient (−Fe) medium for 8 d, and then shifted to iron-sufficient medium for 30 min (30′), 1 h (1H), or 18 h (18H). A, Ribonuclease protection assay. ys1 indicates the position of signal due to specific ribonuclease protection of the Ys1-specific probe; tub indicates the position of signal due to specific ribonuclease protection of the tubulin probe, which serves as an internal loading control. B, Immunoblot using anti-YS1 antibodies.