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Functional complementation of fet3fet4 yeast. DEY1453-derived yeast strains transformed with constructs expressing maize Ys1, AtIRT1, or the empty vector, pFL61, were grown on synthetic defined medium containing: A, 50 μm iron citrate; B, 10 μm Fe(III)Cl3; C, 10 μm Fe(III)Cl3 with 10 μm MA; D, 4 μm Fe(II)SO4; E, 4 μm Fe(II)SO4 with 5 μm NA; F, 10 μm Fe(III)Cl3 with 10 μm NA. Pairs of spots correspond to 10-fold and 100-fold dilutions of the original cultures.
