Fig. 3.

Effects of EPA on LPA-induced phosphorylation of p70S6K in DU145 and Caco-2 cells. (A) Serum-starved DU145 cells were incubated with or without 20 µM EPA for 15 minutes. LPA (10 µM) was then added for the indicated times. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-p70S6K (p-p70S6K) and for actin (loading control). (B) Immunoblotting results were quantified from experiments in which cells were incubated for the indicated times with EPA, LPA, or EPA plus LPA. Results were normalized to actin, and then to the untreated control. Each value represents the mean ± S.E.M. from duplicate experiments, except for EPA alone, which was from a single experiment. *Values that are significantly different from untreated control. (C) Serum-starved Caco-2 cells were incubated with or without 20 µM EPA or 10 µM LPA for the indicated times. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-p70S6K and for actin (loading control). Immunoblotting results were quantified from three separate experiments. Results were normalized to actin, and then to the untreated control. Each value represents the mean ± S.E.M. from duplicate experiments. When there were two separate experiments for the same time point, the line on the graph was drawn through the mean of the data points. *P < 0.05.