Fig. 3.

Disruption of mitochondrial homeostasis in endotoxic AKI. Total RNA and DNA were harvested from renal cortical tissue of mice treated with saline vehicle or LPS (10 mg/kg i.p.) at 1, 3, 18, and 42 hours. (A) Expression of key regulators of mitochondrial biogenesis (NRF-1, TFAM), nuclear-encoded components of the electron transport chain (NDUFS1, NDUFB8, ATPSβ), and mitochondrial-encoded respiratory proteins (COX1, ND1) were measured at the mRNA level (n = 6/group). (B) Relative mitochondrial DNA content in the renal cortex was determined by qRT-PCR analysis (n = 5–6 animals/group). Data are shown as expression relative to vehicle-treated animals (mean ± S.E.M.). *P < 0.05 versus time point controls.