Fig. 7.

The relationship between changes in mitochondrial respiration, let-7f, and histone acetylation. The levels of mitochondrial respiration, let-7f level, and histone acetylation were measured in the prefrontal cortex of rats using the method of XF respirometry, gold immunolabeling of acetylated histones proteins, and TaqMan let-7f primers, respectively. The data were analyzed using a Pearson correlation statistical assay to determine a physiologic relationship between changes in the three variables. The degree of mitochondrial respiration was inversely correlated with that of let-7f (r = −0.4, P = 0.007) and histone acetylation (r = −0.49, P < 0.0001), but no correlation was found between the level of let-7f and histone acetylation. Depicted are multiple data points from 14 rats.