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Diagram of the in situ perfusion system. The liver is continuously perfused with a solution containing 100 μg/ml cefmetazole, which enters the liver via the portal vein. The inferior vena cava (IVC) is ligated, so the perfusate exits via the superior vena cava (SVC) after passing through the liver. The common bile duct is cannulated to direct all bile flow to the gallbladder, which is where the bile is collected.
