. 2015 Feb;87(2):197–206. doi: 10.1124/mol.114.095471

TABLE 4.

LDK1229 binding to the wild-type or mutant CB₁ receptors using [³H]CP55,940 or [³H]WIN55,212-2 as a tracer

Receptor	[³H]CP55,940
Receptor	K_i^a	K_i Ratio^b
	nM
CB₁ wild type	220 (106–457)	1:1
K3.28¹⁹²A	1316 (1115–1626)^c	1:4^c
W5.43²⁷⁹A	ND	—
W6.48³⁵⁶A	1987 (895–2736)	1:9
C7.42³⁸⁶M	1010 (832–1154)	1:5

ND, not detectable; 39% displacement at 32 µM.

^{^a}

Data are the median and corresponding 95% confidence limits of three independent experiments performed in duplicate. K_i values were determined from competition binding assays using [³H]CP55,940 as tracer at its respective K_d values, as described in Results.

^{^b}

Wild type:mutant.

^{^c}

Binding was performed with [³H]WIN55,212-2 as a tracer. LDK1229 binding to CB₁ wild type using [³H]WIN55,212-2 as a tracer yielded a K_i of 324 nM (205–524).