Fig. 5.
EPAC1 facilitates activation of integrin β1. Cells were treated with 007-AM in the presence or absence of ESI-09 or NPC 15437, and activation of integrin β1 was probed by FACS using the antibody 12G10, which only binds to the active form of integrin β1. Total integrin β1 was probed with the antibody K-20. (A) A representative histogram showing the binding of 12G10. (B) Quantification of active Itgβ1 relative to total Itgβ1 [mean fluorescence intensity (MFI)active/MFItotal]. **Significantly higher than vehicle-treated Ctrl cells (P < 0.03). *Significantly lower than vehicle-treated Ctrl cells (P < 0.04). Bars represent mean ± S.D. (n = 3).