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d-Limonene enhances mda-7/IL-24–mediated apoptosis. (A) LT2, (B) MIA PaCa-2, and (C) BxPC-3 cells were treated with NAC prior to treatment with Ad.5/3-mda-7 (m-7) (25 pfu/cell) and d-limonene (D-L) (100 μM). Apoptosis was measured after 48 hours by Annexin V/PI staining and flow-cytometer analysis. Western blotting was performed from the whole-cell lysates 48 hours post-treatment. V, Annexin V.
