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. 2015 Feb;87(2):286–295. doi: 10.1124/mol.114.094375

Fig. 7.

Fig. 7.

d-Limonene (d-Lim) enhances the binding of weakly translated mda-7/IL-24 mRNA with polysomes. (A) MIA PaCa-2 cells were infected with Ad.5-mda-7 or Ad.5/3-mda-7 (25 pfu/cell) and subsequently treated with 100 μM d-Lim. Polysomal fractions were separated using a 10–40% sucrose gradient as indicated by graph. Fractions were pooled into two sets, i.e., fraction I (1–10 fraction number) and fraction II (enriched with mRNA associated with polysomes). (B) mRNA was isolated and reverse transcription PCR was performed using a probe for mda-7/IL-24 and GAPDH. (C) The relative quotient of mda-7/IL-24 with respect to GAPDH. (D) Western blotting was performed on lysates from cells infected with Ad.5-mda-7 or Ad.5/3-mda-7 (25 pfu/cell), with or without d-Lim and/or NAC, using antibodies for preinitiation complex markers and MDA-7/IL-24. Level of significance: **P < 0.01; ***P < 0.001.