Figure 3.
Generation of the recombinant pMal-Cah6 expression construct. A, A schematic figure showing the alignment of primers used to amplify Cah6 cDNA. PCR primers are denoted by black arrows. SP2 and R4H primers were used to amplify a cDNA PCR product that codes for the full-length protein while MP and R4H primers were used to amplify a cDNA product that codes for the putative mature protein. R4H primer has a HindIII site incorporated at the 5′ end. The numbers on the top of the primers denote the primer position in bp. B, A part of the pMal vector showing the polylinker cloning site (PCS),β-galactosidase (LacZα), and β-lactamase (Ampr) genes, XmnI and HindIII recognition sites. Xa denotes Factor Xa cleavage site. MalE codes for MBP. C, A schematic figure of the pMal-Cah6 recombinant construct.