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A western blot probed by the Cah6 antibody using purified overexpressed MBP-Cah6 protein. Lane 1, 20 μg of purified undigested fusion protein; lane 2, 20 μg of fusion protein cleaved by 1 μg of Factor Xa; lane 3, 1 μg of Factor Xa. ST represents prestained low molecular mass markers.
