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. 2015 Feb;87(2):338–348. doi: 10.1124/mol.114.095745

Fig. 3.

Fig. 3.

Negative-gating modulation of endogenous KCa3.1 and KCa2.3 channels in porcine endothelium and RA-2–evoked modulation of arterial contraction and relaxation of the PCA. (A) Left panel: potentiation of KCa2/KCa3.1 from preactivation levels (during infusion of 1 µM Ca2+) by 1 µM SKA-31 followed by current inhibition by 1 µM RA-2. Middle panel: partial inhibition of SKA-31–potentiated current by 1 µM TRAM-34 followed by complete inhibition of the TRAM-34–insensitive component by 1 µM RA-2. Right panel: summary data. Data points are mean ± S.E.M. (n = 3-8, arteries for each compound). *P < 0.01 SKA-31 versus Ctrl (without compounds); #P < 0.05; SKA-31 versus TRAM-34, RA-2, or TRAM-34 and RA-2; Student’s t test. (B) RA-2 inhibited BK (1 µM)-induced relaxation in rings strongly precontracted with U46619 (0.2 µM). Data points are mean ± S.E.M. (n = 5–6, arteries each); **P < 0.01.