Fig. 2.

Genomic localization of lmo0171 gene and a model of used mutagenization. The lmo0171 fragment was amplified with primers p171L and p171R and cloned into Xba I site of pKSV7 plasmid. The resultant vector was electroporated to the competent L. monocytogenes cells and chloramphenicol-resistant transformants were selected. Several restriction enzyme cleavage sites and the locations of lmo0171 and other essential sequences are indicated (amp—ampicillin resistance gene, cm—chloramphenicol resistance gene, lacZ—beta-galactozidase gene, pE194TS—thermosensitive replication origin)