Table 1.
Human primers sequences
| gene symbol | forward primer (5’→ 3’) | reverse primer (5’→ 3’) | product length (bp) |
|---|---|---|---|
| ALPL | GGCTTCTTCTTGCTGGTGGA | CAAATGTGAAGACGTGGGAATGG | 181 |
| COL1A1 | TGAGCCAGCAGATCGAGA | ACCAGTCTCCATGTTGCAGA | 178 |
| GAPDH | TCAACAGCGACACCCAC | GGGTCTCTCTCTTCCTCTTGTG | 203 |
| OCN | GCAGCGAGGTAGTGAAGAGAC | AGCAGAGCGACACCCTA | 193 |
| ON | TGCATGTGTCTTAGTCTTAGTCACC | GCTAACTTAGTGCTTACAGGAACCA | 183 |
| OPN | TGGAAAGCGAGGAGTTGAATGG | GCTCATTGCTCTCATCATTGGC | 192 |
| PPARG | CAGGAGATCACAGAGTATGCCAA | TCCCTTGTCATGAAGCCTTGG | 173 |
| RUNX2 | AGCCTTACCAAACAACACAACAG | CCATATGTCCTCTCAGCTCAGC | 175 |
ALPL, alkaline phosphatase, liver/bone/kidney, COL1A1, collagen, type I, alpha 1, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, OCN, osteocalcin, ON, osteonectin, OPN, osteopontin , PPARG, peroxisome proliferator-activated receptor gamma, RUNX2, runt- related transcription factor 2