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. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Cell Signal. 2013 Jul 6;25(11):2163–2175. doi: 10.1016/j.cellsig.2013.07.002

Figure 6. Aldosterone induces MAPKs phosphorylation in ΔKinase and K1648R but not in WT hTRPM7 cells.

Figure 6

HEK-293 cells expressing WT hTRPM7 channels or the mutants ΔKinase (deleted kinase) and K1648R (inactive kinase) were stimulated with 100 nM aldosterone (1- 60 min). Line graphs, effect of aldosterone on (A) p38MAPK, (B) SAPK/JNK MAPK, and (C) ERK1/2 MAPK phosphorylation. Results are expressed as % of vehicle (mean ± S.E.) of 7 independent experiments. Side panels, representative immunoblots of p38MAPK [Thr180/Tyr182], p38MAPK, SAPK/JNK MAPK [Thr183/Tyr185], SAPK/JNK MAPK, ERK1/2 MAPK [Thr202/Tyr204], ERK1/2 MAPK. *p<0.05 versus corresponding stimulation time in HEK-293 cells expressing WT hTRPM7.