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. 2014 Jul;93(7):691–697. doi: 10.1177/0022034514533126

Figure 2.

Figure 2.

Myofibroblastic differentiation. (A) Serum-starved young and old HGFs were stimulated with 10 ng/mL TGF-β1 for 72 hrs. Actin cytoskeleton distribution and α-SMA staining were evaluated through immunofluorescence. Scale bar equals 10 μm. (B) Graph indicates average ± standard error of α-SMA (+) cells normalized against total cells. Asterisk indicates a statistically significant difference between young group compared with all other conditions (p < .0001, Kruskal-Wallis test). (C) Serum-starved young and aged HGFs were stimulated with 10 ng/mL TGF-β1 for 72 hrs, and α-SMA were analyzed through Western blot of the cell lysates. Graph represents the average ± standard error of 4 independent experiments from different cell lines. Asterisks indicate a statistically significant difference between young and old fibroblasts in both resting and stimulated (TGFβ1) conditions.