Figure 2.

MBP-kinase activity during adventitious root development induced by NO. A, Cucumber explants were incubated for different times with water or 10 μm of the NO-donor SNP or with SNP plus 200 μm of the specific NO-scavenger cPTIO. In vitro MBP-kinase activity was measured in total soluble extracts from those explants. Data are means of two independent experiments with three repetitions for each point. The sd of water and SNP plus cPTIO-treatments are not shown for the sake of clarity of the figure. B, In-gel protein kinase assays. The arrow heads indicate an apparent molecular mass of approximately 48 kD.