Figure 3.

MBP-kinase activity during the IAA-induced adventitious root development requires endogenous NO. A, Cucumber explants were incubated for different times either with 10 μm IAA or with IAA plus 200 μm of the specific NO-scavenger cPTIO. In vitro MBP-kinase activity was measured in total soluble extracts from those explants. Values are expressed as mean ± sd from two independent experiments with three repetitions for each point. B, Protein kinase activity was measured by in-gel assays as described. The arrow heads indicate an apparent molecular mass of approximately 48 kD.