Figure 5.

MAPK activity is required for adventitious root formation induced by IAA and NO. A, Cucumber explants were treated for 5 d as indicated. Root number values are expressed as mean ± se (n = 10 explants from at least 3 independent experiments). Bars with different letters are significantly different with P < 0.05 (t test). The cell-permeable MAPKK inhibitor PD098059 was used at 50 μm and SNP and IAA were used at 10 μm. The inset shows the effect of different concentrations of PD098059 on the NO-induced adventitious root formation. Cucumber explants were treated with water (control) or 10 μm SNP in the presence or absence of 10 μm, 50 μm, or 100 μm of the MAPKK inhibitor PD098059 for 4 d. Inhibition values are expressed as %, where 100% means root number values per explant similar to those obtained in control explants. B, Photographs were taken after 4 d of treatment. Bar indicates 5 mm.