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. 2014 Jul;93(7):678–684. doi: 10.1177/0022034514535214

Figure 4.

Figure 4.

The knockdown of NOD2 reversed MDP-mediated osteoclastogenic cytokine expression in HDPCs. (A, B) Control siRNA- or NOD2 siRNA-transfected HDPCs were cultured in the presence of MDP (10 µg/mL) for 7 days in osteogenic media (OM). The mRNA levels of osteoclastogenic cytokines were evaluated by RT-PCR analysis. (C) The osteoclastogenic activity of conditioned medium (CM) from NOD2-silenced HDPCs was assessed by a mouse osteoclast differentiation system. HDPCs were transfected with either control siRNA or NOD2 siRNA and subsequently treated with MDP (10 µg/mL) for 14 days. The CM was collected as described in Materials & Methods and treated on pre-osteoclast cells. After 2 days, the cultures were stained for TRAP activity. (D) The numbers of osteoclasts/well were counted. *Statistically significant difference vs. control, p < .05. #Statistically significant difference in OM alone, p < .05. Similar data were obtained from 3 independent experiments.