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. 2014 Aug;93(8):807–812. doi: 10.1177/0022034514536730

Figure 2.

Figure 2.

Changes of tyrosine hydroxylase (TH) immunoreactivity in the periodontal ligament area on the compression side adjacent to the mesial root of the maxillary first molar; also, osteoclast activity on the compression side and bone formation parameter on the tension side along the alveolar bone 1 mo after spring insertion. Rats received an injection of calcein (15 mg/kg, intraperitoneal) 10 and 3 d before the end of the experiment. (A) Representative pictures of immunohistochemical photomicrographs in periodontal ligament area. Bar = 50 µm. (B) Comparison of changes in TH-positive area (* p < .05; *** p < .001). (C) Representative microscopic photographs of tartrate-resistant acid phosphatase (TRAP)–stained histologic sections of the alveolar bone. Bar = 50 µm. (D) Comparison of changes in osteoclast surface/bone surface (Oc.S/BS) (** p < .01; *** p < .001). (E) Representative fluorescent micrographs of undecalcified sections of the alveolar bone. Double labeling lines of calcein. Bar = 20 µm. (F) Comparison of changes in bone formation rate per bone surface (BFR/BS) (*** p < .001).